Application of Metal-Coded Affinity Tags (MeCAT): Absolute Proteir Quantification with Top-Down and Bottom-Up Workflows by Metal Coded Tagging

• Published in: Analytical chemistry (Washington) Vol. 84; no. 12; pp. 5268 - 5275
• Main Authors: BERGMANN, U, AHRENDS, R, NEUMANN, B, SCHELER, C, LINSCHEID, M. W
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 2012
• Subjects: Analytical chemistry; Biological and medical sciences; Chemistry; Diverse techniques; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Molecular and cellular biology; Spectrometric and optical methods; Cysteine; Lanthanide; Peptides; Internal standard; Metal; Inductive coupling plasma spectrometry; Electrospray; Reporter gene; Fusion protein; β-Galactosidase; Quantitative analysis; Flow injection; Enzyme; Coupled method; Sample; Chemical ionization; Glycosylases; Enzymatic method; Residue; Glycosidases; Mass spectrometry MS/MS; Proteomics; Chelating agent; Tagging; Hydrolases; Affinity; Strategy; Yield; Two dimensional electrophoresis; Application; Mass spectrometry
• ISSN: 0003-2700; 1520-6882