PPAR-{gamma} agonist protects against intestinal injury during necrotizing enterocolitis

• Published in: Biochemical and biophysical research communications Vol. 379; no. 2
• Main Authors: Baregamian, Naira, Mourot, Joshua M., Ballard, Amie R., Evers, B. Mark, Sealy Center for Cancer Cell Biology, University of Texas Medical Branch, Galveston, TX 77555, Chung, Dai H.
• Format: Journal Article
• Language: English
• Published: United States 06-02-2009
• Subjects: 60 APPLIED LIFE SCIENCES; ARTERIES; HORMONES; INFLAMMATION; INJURIES; ISCHEMIA; MICE; RECEPTORS; SMALL INTESTINE
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2008.11.155

Necrotizing enterocolitis (NEC) remains a lethal condition for many premature infants. Peroxisome proliferator-activated receptor-{gamma} (PPAR-{gamma}), a member of the nuclear hormone receptor family, has been shown to play a protective role in cellular inflammatory responses; however, its role in NEC is not clearly defined. We sought to examine the expression of PPAR-{gamma} in the intestine using an ischemia-reperfusion (I/R) model of NEC, and to assess whether PPAR-{gamma} agonist treatment would ameliorate I/R-induced gut injury. Swiss-Webster mice were randomized to receive sham (control) or I/R injury to the gut induced by transient occlusion of superior mesenteric artery for 45 min with variable periods of reperfusion. I/R injury resulted in early induction of PPAR-{gamma} expression and activation of NF-{kappa}B in small intestine. Pretreatment with PPAR-{gamma} agonist, 15d-PGJ{sub 2}, attenuated intestinal NF-{kappa}B response and I/R-induced gut injury. Activation of PPAR-{gamma} demonstrated a protective effect on small bowel during I/R-induced gut injury.