Quantitative expression patterns of peroxisome proliferator-activated receptor-{beta}/{delta} (PPAR{beta}/{delta}) protein in mice

• Published in: Biochemical and biophysical research communications Vol. 371; no. 3
• Main Authors: Girroir, Elizabeth E., Hollingshead, Holly E., Graduate Program in Biochemistry, Microbiology, and Molecular Biology, Pennsylvania State University, University Park, PA 16802, He Pengfei, Zhu Bokai, Perdew, Gary H., Peters, Jeffrey M.
• Format: Journal Article
• Language: English
• Published: United States 04-07-2008
• Subjects: 60 APPLIED LIFE SCIENCES; ANTIBODIES; BIOCHEMISTRY; BRAIN; EPITHELIUM; HEART; IN VITRO; LARGE INTESTINE; LIVER; LUNGS; MICE; MUSCLES; RECEPTORS; SMALL INTESTINE; SPLEEN; THYMUS
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2008.04.086

The expression patterns of PPAR{beta}/{delta} have been described, but the majority of these data are based on mRNA data. To date, there are no reports that have quantitatively examined the expression of PPAR{beta}/{delta} protein in mouse tissues. In the present study, a highly specific PPAR{beta}/{delta} antibody was developed, characterized, and used to examine tissue expression patterns of PPAR{beta}/{delta}. As compared to commercially available anti-PPAR{beta}/{delta} antibodies, one of six polyclonal anti-PPAR{beta}/{delta} antibodies developed was significantly more effective for immunoprecipitation of in vitro-translated PPAR{beta}/{delta}. This antibody was used for quantitative Western blot analysis using radioactive detection methods. Expression of PPAR{beta}/{delta} was highest in colon, small intestine, liver, and keratinocytes as compared to other tissues including heart, spleen, skeletal muscle, lung, brain, and thymus. Interestingly, PPAR{beta}/{delta} expression was localized in the nucleus and RXR{alpha} can be co-immunoprecipitated with nuclear PPAR{beta}/{delta}. Results from these studies demonstrate that PPAR{beta}/{delta} expression is highest in intestinal epithelium, liver, and keratinocytes, consistent with significant biological roles in these tissues.