Histochemistry of Six Lections in the Tissues of the Flat fish Paralichthys olivaceus

• Published in: Journal of veterinary science (Suwŏn-si, Korea) pp. 293 - 301
• Main Authors: Tae-KyunShin, Mee-JungAhn, Yong-DukLee, Gyung-MinGo, Kyung-SookJung
• Format: Journal Article
• Language: Korean
• Published: 대한수의학회 01-12-2002
• Subjects: 수의학
• ISSN: 1229-845X; 1976-555X

Lectins are glycoproteins that specifically bindcarbohydrate structures and may participate in thebiodefense mechanisms of fish. In this study, thebinding of three lectins, Dolichos biflorus agglutinin(DBA), soybean agglutinin (SBA), Bandeiraea sim-plicifolia BS-1 (isolectin B4),Triticum vulgaris (WGA),Arachis hypogaea (PNA) and Ulex europaeus (UEA-I)were studied in the gill,liver,intestine,kidney,heart,and spleen of the flat fish Paralichthys olivaceus.DBA was detected in intestinal mucous cells, as wellas in gill epithelial and mucous cells. It was weaklydetected in renal tubule epithelial cells and in bileduct epithelial cells. The strong SBA staining wasseen in the intestinal club cells,in bile duct epithelialcells and renal tubule epithelial cells. There wereintense positive reactions for isolectin B4 in gillepithelial and mucous cells, and the strong isolectinB4 staining was seen in epithelial cells of the bileduct and intestine.The strong WGA staining was seenin the gill mucosal cells, sinusoid, renal tubuleepithelial cells and mucosal cells of the intestine.UEA-I was detected in the gill epithelial and mucosalcells, bile duct epithelial cells and renal tubularepithelial cells.These results suggest that the six lectins examinedwere localized in the covering epithelia of the variousorgans of the flat fish and they may participate in thebiodefense mechanism of the intra body surface inwhich is exposed to various antigens. KCI Citation Count: 0