Enhanced Saccharification of Rice Straw Using Hypochloritehydrogen Peroxide

• Published in: Biotechnology and bioprocess engineering pp. 273 - 281
• Main Authors: 강희경, Nahyun M. Kim, Ghahyun J. Kim, Eun-Seong Seo, Hwa-Ja Ryu, Sang-Il Yun, Hyun-Chul Choi, Donal F. Day, Jongho Kim, Dong-Lyun Cho, 김도만
• Format: Journal Article
• Language: English
• Published: 한국생물공학회 01-04-2011
• Subjects: 생물공학
• ISSN: 1226-8372; 1976-3816

Rice straw is a lignocellulosic biomass, and has been recognized as a renewable organic substance and alternative energy source. In this study, rice straw was pretreated with hypochlorite-hydrogen peroxide (Ox-B)solution. The optimal pretreatment conditions were determined via response surface methodology, and the pretreated rice straw was hydrolyzed with exo-glucanase, endoglucanase,hemicellulase, and β-glucosidase Accellerase 1000™ (endo-glucanase equivalent activity of 1,250 carboxy methyl cellulose (CMC) U/g of rice straw pretreated for 24h). The optimal conditions were as follows: 60 min pretreatment using Ox-B solution containing 0.6% hypochlorite and 25% hydrogen peroxide for 1 g of rice straw in a total reaction volume of 240 mL. Under these conditions, 406.8mg of D-glucose and 224.0 mg of D-xylose were obtained from 1 g of rice straw. The fermentation of enzymatic hydrolysates containing 8.14 g/L D-glucose and 4.49 g/L D-xylose with Pichia stipitis generated 3.65 g/L of ethanol with a corresponding yield of 0.37 g/g. The maximum possible ethanol conversion rate is 72.54%. KCI Citation Count: 11