Cloning, Purification, and Characterization of a New DNA Polymerase froma Hyperthermophilic Archaeon, Thermococcus sp. NA1

Genomic analysis of Thermococcus sp. NA1revealed the presence of a 3,927-base-pair (bp) family B-typeDNA polymerase gene, TNA1_polintein, was overexpressed in Escherichia coli, purified usingmetal affinity chromatography, and characterized. TNA1_polactivity was optimal at pH 7.5 and 75oC. TNA1_pol w...

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Bibliographic Details
Published in:	Journal of microbiology and biotechnology pp. 1090 - 1097
Main Authors:	KIM, YUN JAE, HYUN SOOK LEE, SEUNG SEOB BAE, JEONG HO JEON, 임재규, YONA CHO, KI HOON NAM, SUNG GYUN KANG, SANG-JIN KIM, SUK-TAE KWON, JUNG-HYUN LEE
Format:	Journal Article
Language:	Korean
Published:	한국미생물·생명공학회 01-07-2007
Subjects:	생물학
Online Access:	Get full text
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Summary:	Genomic analysis of Thermococcus sp. NA1revealed the presence of a 3,927-base-pair (bp) family B-typeDNA polymerase gene, TNA1_polintein, was overexpressed in Escherichia coli, purified usingmetal affinity chromatography, and characterized. TNA1_polactivity was optimal at pH 7.5 and 75oC. TNA1_pol washighly thermostable, with a half-life of 3.5 h at 100oC and12.5 h at 95oC. Polymerase chain reaction parameters ofTNA1_pol such as eror-rate, procesivity, and extension ratewere measured in comparison with rTaq, Pfu, and KOD DNA4.45 kilobases (kb), and had a processivity of 150 nucleotides(nt) and an extension rate of 60 bases/s. Thus, TNA1_pol hasa much faster elongation rate than Pfu DNA polymerase with7-fold higher fidelity than that of rTaq. KCI Citation Count: 26
Bibliography:	G704-000169.2007.17.7.015
ISSN:	1017-7825