Effect of Concentration and Exposure Duration of FBS on Parthenogenetic Development of Porcine Follicular Oocytes

• Published in: Journal of animal reproduction & biotechnology (Online) pp. 245 - 249
• Main Authors: Hyun-Jong Kim, Sang-Rae Cho, Chang-Yong Choe, Sun-Ho Choi, Dong-Soo Son, Sung-Jae Kim, Byung-Don Sang, Man-Hye Han, Il-Sun Ryu, In-Cheul Kim, Il-Hwa Kim, Woon-Kyu Lee, Kyung-Soon Im
• Format: Journal Article
• Language: Korean
• Published: 사단법인 한국동물생명공학회 01-12-2007
• Subjects: 축산학
• ISSN: 2671-4639; 2671-4663

The aim of present experiment was to examine hatching rate as in vitro indicator of viability of porcine embryos before early stage embryo transfer such as zygotes or 2-cell stage embryos. Cumulus-oocyte complexes (COCs) collected from ovaries were matured in North Carolina State University 23 (NCSU-23) containing 10% porcine follicular fluid (pFF), 10 ng/ml epidermal growth factor (EGF), 10μg/ml follicle stimulating hormone (FSH), 35μg/ml luteinizing hormone (LH), and 1mg/ml cysteine. After 24 hours, the COCs were transferred to the same medium without hormones. After 65h of maturation, oocytes were exposed to phosphate buffered saline (PBS) with 7% ethanol (v/v) for 7 minutes, and then the oocytes were washed and cultured in tissue culture medium (TCM) 199 containing 5 ug/ml cytochalasin B for 5h at 38.5℃ in an atmosphere of 5% CO2 and 95% air with high humidity. After cytochalasin B treatment, the presumptive parthenotes were cultured in porcine zygote medium (PZM)-5 and cleavage of the parthenotes was assessed at 72h of activation, Normally cleaved parthenotes were cultured for an additional 8 days to evaluate their ability to develop to blastocyst and hatching stages. The fetal bovine serum (FBS) were added at Day 4 or 5 with concentrations of 2.5, 5 or 10%. The blastocyst rates were ranged within 39.1～70% in each treatment. However hatching rate was dramatically decreased in non-addition group. In this experiment, embryo viability in female reproductive tract may be estimated before embryo transfer with in vitro culture adding FBS by hatching ability. KCI Citation Count: 0