Anticholinesterase and Anti-inflammatory Constituents from Beilschmiedia pulverulenta Kosterm

• Published in: Natural product sciences Vol. 22; no. 4; pp. 225 - 230
• Main Authors: Wan Salleh, Wan Mohd Nuzul Hakimi, Ahmad, Farediah, Yen, Khong Heng, Zulkifli, Razauden Mohamed
• Format: Journal Article
• Language: Korean
• Published: 2016
• Subjects: Beilschmiedia pulverulenta; Anticholinesterase; Lignan; Anti-inflammatory
• ISSN: 1226-3907

Phytochemical investigation from the stem bark of Beilschmiedia pulverulenta resulted in the isolation of five lignans, (+)-yangambin (1), (+)-sesartemin (2), (+)-excelsin (3), (+)-sesamin (4), and (+)-syringaresinol (5), together with lupeol (6), lupenone (7), ${\beta}-sitosterol$ (8), and ${\beta}-sitostenone$ (9). Their structures were established by the analysis of their spectroscopic (1D and 2D NMR) and spectrometric (MS) data, as well as by comparison with those reported in the literature. The isolated lignans were tested for their anticholinesterase (AChE: acetylcholine esterase and BChE: butyryl cholineesterase) and anti-inflammatory (COX-2: cyclooxygenase-2 and LOX: lipoxygenase) activities. All the isolated lignans (1 - 5) exhibited significant inhibition activities in AChE/BChE and COX-2/LOX assays with $IC_{50}$ values ranging from $168.8-504.2{\mu}M$ and $21.0-59.4{\mu}M$, respectively.