Comparative Analysis of Cutaneous Fungi in Atopic Dermatitis Patients and Healthy Individuals

Comparative Analysis of Cutaneous Fungi in Atopic Dermatitis Patients and Healthy Individuals

Background: Atopic dermatitis (AD) is a chronic relapsing inflammatory skin disease triggered by diverse factors. Microbes are one of the crucial risk factors for AD development or exacerbation. However, the effect of a fungal burden on AD has been overlooked compared to bacteria. Objective: This st...

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Bibliographic Details
Published in:Annals of dermatology Vol. 34; no. 2; pp. 118 - 124
Main Authors: Yoojeong Choi, Kui Young Park, Hye Sung Han, Mi-kyung Lee, Seong Jun Seo
Format: Journal Article
Language:Korean
Published: 대한피부과학회 05-04-2022
Subjects:
Arthrodermataceae
Atopic dermatitis
Candida
Fungi
Malassezia
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Summary:Background: Atopic dermatitis (AD) is a chronic relapsing inflammatory skin disease triggered by diverse factors. Microbes are one of the crucial risk factors for AD development or exacerbation. However, the effect of a fungal burden on AD has been overlooked compared to bacteria. Objective: This study aimed to comparatively analyze cutaneous fungal distribution between AD patients and healthy individuals by polymerase chain reaction (PCR)-based analysis. Methods: Skin samples of AD outpatients and healthy individuals collected at the Chung-Ang University were analyzed. Representative AD-associated fungal genera, Candida, dermatophytes, and Malassezia, were analyzed using specific primer and amplification methods. Amplicons were sequenced, and the fungal distribution of both groups were compared. Results: Totally, 211 patients and 23 healthy individuals were studied. Of the 211 patients, 10.90% (23/211) had Candida species, whereas 0% (0/23) healthy individuals showed its presence. The most frequently detected species in patients was Candida albicans (5.21%) followed by Candida parapsilosis (3.79%). For dermatophytes, 1.42% (3/211) of patients showed positive results, whereas 0% (0/23) healthy individuals showed positive results. Malassezia species were identified in 20.85% (44/211) and 8.70% (2/23) in patients and healthy individuals, respectively. Malassezia restricta was the most frequently identified species in the AD patient group, and the only species found in the healthy control group. Conclusion: The distribution of Candida spp., dermatophytes, and Malassezia spp. are altered with AD development.
Bibliography:The Korean Dermatological Association
ISSN:1013-9087
2005-3894