Crisscross Enzymatic Reaction between the Two Molecules in the Active Dimeric P69 Form of the 2′-5′ Oligodenylate Synthetase

• Published in: The Journal of biological chemistry Vol. 277; no. 47; p. 44760
• Main Authors: Saumendra N. Sarkar, Srabani Pal, Ganes C. Sen
• Format: Journal Article
• Language: English
• Published: American Society for Biochemistry and Molecular Biology 22-11-2002
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M207126200

2′-5′ oligoadenylate (2–5 (A)) synthetases are major components of the antiviral pathways induced by interferons. In the presence
of double-stranded RNA, they polymerize ATP to form 2–5 (A) oligomers that, in turn, activate the latent ribonuclease RNase
L, causing mRNA degradation. These enzymes, unlike other nucleotidyl transferases, catalyze 2′-5′, not 3′-5′, phosphodiester
bond formation between substrates bound to the acceptor and donor sites. Moreover, unlike other members of this extended family,
the P69 isozyme of 2–5 (A) synthetase functions as a homodimer. Here, we report that the need for P69 dimerization is because
of a crisscross enzyme reaction joining two substrate molecules bound to two opposite subunits. Consequently, although homodimers
of mutants in the previously identified acceptor site, the donor site, or the catalytic site were inactive, selective heterodimers
of the mutants were active because of subunit complementation. The catalytic site had to be present in the same subunit that
contained the acceptor site, whereas the donor site had to be provided by the other subunit. These results allowed us to design
a mutant protein that acted as a dominant-negative inhibitor of wt P69 but not of another isozyme of 2–5 (A) synthetase.