Inflammatory biomarkers and oxidative stress indicators in bitches subjected to conventional ovarysalpingohysterectomy, by hybrid NOTES and by total NOTE/ Biomarcadores inflamatorios e indicadores de estresse oxidativo em cadelas submetidas a ovariossalpingohisterectomia convencional, por NOTES hibrida e NOTES total

• Published in: Ciência rural Vol. 44; no. 5; p. 884
• Main Authors: Basso, Paula Cristina, Raiser, Alceu Gaspar, Brun, Mauricio Veloso, Mazzanti, Cinthia Melazzo, Costa, Marcio Machado, Wolkmer, Patricia, Dutra, Luciana Hermes, Muller, Daniel Curvello de Mendonca, Silva, Cassia Bagolin
• Format: Journal Article
• Language: Spanish
• Published: Universidade Federal de Santa Maria 01-05-2014
• Subjects: Analysis; Antioxidants; Lipid peroxidation
• ISSN: 0103-8478

This study aimed at comparing the inflammatory response and the oxydative profile of the conventional ovarysalpingohysterectomy (OSH) technique to Totally Natural Orifice Transluminal Endoscopic Surgery (NOTES) and Hybrid NOTES. Group of fifteen female dogs was used for each technique. Blood samples were taken before the surgical procedure (basal) and 3, 6, 12, 24, 48 and 72h postoperative. The catalase activity was increased in the three studied groups. Nevertheless, lipid peroxidation, measured by TBARS (thiobarbituric acid reactive substances) levels, was higher in the conventional group and almost indistinguishable in the total NOTES group. In the three analyzed groups, both butyrylcholinesterase and acetylcholinesterase activities were increased as well as the neutrophil counts during the first post-surgical hours. It is possible to conclude that systemic inflammation occurs in a similar way in the three operative techniques; however, total NOTES technique presents lower levels of cellular oxidative damage, particularly if compared to the conventional approach. Key words: butyrylcholinesterase, acetylcholinesterase, lipid peroxidation, antioxidant enzymes, scarless surgery. Objetivou-se comparar a resposta inflamatoria e o perfil oxidativo da tecnica de ovariossalpingohisterectomia (OSH) convencional com duas tecnicas de Cirurgia Endoscopica por Orificios Naturais (NOTES). Foram utilizados 15 femeas, alocadas em tres grupos de cinco animais. No primeiro grupo, a OSH foi realizada por celiotomia convencional, no segundo, por NOTES total e, no terceiro, por NOTES hibrida. Foram realizadas as coletas sanguineas antes do procedimento cirurgico (basal), 3, 6, 12, 24, 48 e 72h pos-operatorias. A atividade da catalase mantevese alta nos tres grupos estudados, entretanto a peroxidacao lipidica, medida pelos niveis dos produtos de reacao com o acido tiobarbiturico (TBARS), ocorreu mais acentuadamente no grupo convencional e foi quase que imperceptivel no grupo de NOTES total. Nos tres grupos estudados, ocorreu elevacao na atividade da butirilcolinesterase e acetilcolinesterases, bem como aumento leucocitario neutrofilico nas primeiras horas pos-cirurgicas. Conclui-se que a inflamacao sistemica acontece de forma similar nas tres tecnicas operatorias, com ressalva para as realizadas por NOTES total que mantiveram as mais baixas taxas oxidativas. Palavras-chave: butirilcolinesterase, acetilcolinesterase, peroxidacao lipidica, enzimas antioxidantes, cirurgia sem cicatriz.