Evaluation of survival bradyrhizobium SPP in soybean seed treated with fungicides, guard and cellular inoculant/Avaliacao da sobrevivencia de bradyrhizobium SPP em sementes de soja tratadas com fungicidas, protetor celular e inoculante

The modern agricultural techniques application has an important role in the maintaining the level of second largest producer and exporter of soybean reached by Brazil. Among this techniques are highlighted the Bradyrhizobium inoculation and seed treatment with fungicides. Studies have shown incompat...

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Published in:Revista Internacional de Ciências
Main Authors: Marks, Bettina Berquo, Bangel, Eliane Villamil, Tedesco, Vanessa, Silva, Sabrina Leticia Couto da, Ferreira, Silviane Barra, Vargas, Rafael, Silva, Gilmario Marques
Format: Journal Article
Language:Spanish
Published: Universidade do Estado do Rio de Janeiro- Uerj 01-06-2013
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Summary:The modern agricultural techniques application has an important role in the maintaining the level of second largest producer and exporter of soybean reached by Brazil. Among this techniques are highlighted the Bradyrhizobium inoculation and seed treatment with fungicides. Studies have shown incompatibility between inoculated bacteria with other practices of seed treatment. For this reason, industries have developed cell additives, which favor the survival of microorganisms, even in the presence of fungicides. This study aims to evaluate the effect of a cell additive in the Bradyrhizobium survival in inoculated and treated with fungicides soybean seeds. The essay was conducted in 2008, in the Microbiological Agriculture Laboratory (MIA), located in FEPAGRO, Porto Alegre, southern Brazil. Soybean seeds were treated with four commercial fungicides (Protreat, Vitavax-Thiram, Derosal Plus e Maxim XL), and inoculated, with or without cell additive, constituting ten treatments. The bacteria survival was evaluate in four times (4, 24, 48 and 72 hours). Data were statistically analyzed by comparing the means of treatments by the technique of Analysis of Variance (ANOVA). For both tests, it was established the significance level of 5%. The results showed that the number of Bradyrhizobium cells was lower in treatments without cell additive. The cell additive showed no effect in the survival maintenance of microorganisms when used with Vitavax-Thiram and Derosal Plus fungicides. They were the most harmful to Bradyrhizobium. Treatments with Protreat and Maxim XL were who best responded to the use of cell additive. More studies are needed for further clarification about the effectiveness of cell additive. Keywords: Inoculant, cell additive, fungicide, bradirrizobi. A aplicacao de tecnicas agricolas modernas tem papel importante na manutencao do patamar de segundo maior produtor e exportador de soja alcancado pelo Brasil. Entre essas tecnicas destacamse a inoculacao com bradirrizobios e o tratamento de sementes com fungicidas. Estudos ja realizados demonstraram incompatibilidade entre as bacterias inoculadas com as demais praticas de tratamento de sementes. Em funcao disso, algumas industrias elaboraram aditivos celulares, que favorecem a sobrevivencia de microorganismos inoculados nas sementes, mesmo em presenca de fungicidas. Esse estudo teve como objetivo avaliar o efeito de um aditivo celular na sobrevivencia de bradirrizobios em sementes de soja inoculadas e tratadas com diferentes fungicidas. O ensaio foi conduzido em 2008 no Laboratorio de Microbiologica Agricola (MIA) da FEPAGRO. Sementes de soja foram tratadas com quatro fungicidas comerciais (Protreat, Vitavax-Thiram, Derosal Plus e Maxim XL), e inoculadas, com ou sem aditivo celular, constituindo dez tratamentos. A sobrevivencia das bacterias foi avaliada em quatro periodos pos-inoculacao (4, 24, 48 e 72 horas). Os dados foram analisados estatisticamente atraves da comparacao entre as medias dos tratamentos pela tecnica de Analise de Variancia (ANOVA) e teste de comparacoes multiplas de medias (Teste de Tukey). Para, ambas as analises, foi estabelecido o nivel de significancia de 5%. Os resultados demonstraram que o numero de celulas de bradirrizobio foi menor nos tratamentos que nao receberam aditivo. O aditivo celular nao demonstrou efeito na manutencao da sobrevivencia dos microrganismos quando utilizado com os fungicidas Vitavax-Thiram e Derosal Plus, que se apresentaram como os mais prejudiciais aos bradirrizobios. Os tratamentos que receberam os fungicidas Protreat e Maxim XL foram os que melhor responderam a utilizacao do aditivo celular. Mais estudos sao necessarios para um maior esclarecimento acerca da eficacia do produto. Palavras-Chave: Inoculante, aditivo celular, fungicida, bradirrizobio.
ISSN:2316-7041
2316-7041