Molecular Phylogenetics of Physa acuta

Physids belong to Class Gastropoda; belong to Phylum Mollusca and being bioindicators, intermediate hosts of parasites and pests hold a key position in the ecosystem. There are three species of Genus Physa i.e. P. fontinalis, Physa acuta and P. gyrina water bodies of Central Punjab and were characte...

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Bibliographic Details
Published in:	Brazilian journal of biology Vol. 83
Main Authors:	Ansari, B, Altaf, J, Ramzan, A, Ahmed, Z, Khalil, S, Qamar, S.U.R, Awan, S.A, Jehangir, K, Khalid, R, Aziz, S, Sultana, T, Sultana, S, Alsamadany, H, Alshamrani, R, Awan, F.S
Format:	Journal Article
Language:	English
Published:	Association of the Brazilian Journal of Biology 01-01-2023
Subjects:	Pakistán
Online Access:	Get full text
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Summary:	Physids belong to Class Gastropoda; belong to Phylum Mollusca and being bioindicators, intermediate hosts of parasites and pests hold a key position in the ecosystem. There are three species of Genus Physa i.e. P. fontinalis, Physa acuta and P. gyrina water bodies of Central Punjab and were characterized on the basis of molecular markers High level of genetic diversity was revealed by polymorphic RAPD, however SSR markers were not amplified. The multivariate analysis revealed polymorphism ranging from 9.09 percent to 50 percent among the three Physid species. Total number of 79 loci were observed for the three species under study and 24 loci were observed to be polymorphic. These RAPD fragment(s) can be developed into co dominant markers (SCAR) by cloning and can be further sequenced for the development of the Physa species specific markers to identify the introduced and native species in Pakistan.
ISSN:	1519-6984
DOI:	10.1590/1519-6984.246984