Ultrastructural and quantitative studies of hemocytes in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Pyralidae)

• Published in: Brazilian archives of biology and technology Vol. 46; no. 2
• Main Authors: Falleiros, Ângela Maria Ferreira(Universidade Estadual de Londrina Centro de Ciências Biológicas Departamento de Histologia), Bombonato, Maria Terezinha Siqueira(Universidade Estadual Paulista), Gregório, Elisa Aparecida(Universidade Estadual Paulista Instituto de Biociências Centro de Microscopia Eletrônica)
• Format: Journal Article
• Language: Portuguese
• Published: Tecpar 2003
• Subjects: Differential Hemocyte Count; Hemocytes; Insect; Lepidoptera; Morphometry; Ultrastructural Morphology
• ISSN: 1516-8913

Six circulating hemocytes cell types from Diatraea saccharalis (Fabricius) (Lepidoptera: Pyralidae) larvae were identified by transmission and scanning electron microscope: prohemocytes (PR), plasmatocytes (PL) granulocytes (GR), spherulocytes (SP), oenocytoids (OE) and vermicytes (VE). The PR was the smallest cell type with a large nucleus, a cytoplasm with few organelles and a homogenous smooth surface. The PL was polymorphic and abundant, with a cytoplasm rich in organelles and a cellular surface with several cytoplasmic projections. The GR was abundant, showing two types of membrane-bounded granules (dense and structutered), glycogen, lipid droplets and a surface with philopodial projections. The SP was a large cell, with a cytoplasm full of intracytoplasmic spherules. The OE was the largest hemocyte type with a large and homogeneous cytoplasm and scarce organelles. The VE was discoid in shape and showed electron-dense granules.
Seis tipos de hemócitos circulantes foram caracterizados na hemolinfa de larvas de Diatraea saccharalis (Fabricius) (Lepidoptera: Pyralidae), através da microscopia eletrônica de transmissão e varredura: prohemócitos (PR), plasmatócitos (PL), granulócitos (GR), esferulócitos (ES), oenocitóides (OE) e vermiformes (VE). O PR foi o menor tipo celular; apresentando um núcleo grande, citoplasma com poucas organelas e superfície lisa e homogênea. O PL era polimórfico e abundante, com citoplasma rico em organelas e superfície celular com várias projeções citoplasmáticas. O GR era abundante, apresentando dois tipos de grânulos envoltos por membrana (denso e estruturado), glicogênio, gotas de lipídio e projeções citoplasmáticas filopodiais. O ES era uma célula grande, com citoplasma carregado de esférulas intracitoplasmáticas. O OE foi o maior tipo de hemócito encontrado, apresentando citoplasma grande, homogêneo e escassas organelas. O VE era discóide e apresentou grânulos elétrons-densos.