Rejuvenation of Sequoia sempervirens by repeated grafting of shoot tips onto juvenile rootstocks in vitro

• Published in: Plant physiology (Bethesda) Vol. 98; no. 1
• Main Authors: Huang, L.C. (Academia Sinica, Nankang, Taipei, Taiwan), Lius, S, Huang, B.L, Murashige, T, Mahdi, E.F.M, Van Gundy, R
• Format: Journal Article
• Language: English
• Published: 01-01-1992
• Subjects: AGE; CRECIMIENTO; CROISSANCE; CULTIVO IN VITRO; CULTURE IN VITRO; EDAD; GREFFAGE; INJERTO; PORTAINJERTOS; PORTE GREFFE; SEQUOIA; TALLO; TIGE
• ISSN: 0032-0889; 1532-2548

Repeated grafting of 1.5-centimeter long shoot tips from an adult Sequoia sempervirens tree onto fresh, rooted juvenile stem cuttings in vitro resulted in progressive restoration of juvenile traits. After four successive grafts, stem cuttings of previously adult shoots rooted as well, branched as profusely, and grew with as much or more vigor as those of seedling shoots. Reassays disclosed retention for 3 years of rooting competence at similar levels as originally restored. Adventitious shoot formation was remanifested and callus development was depressed in stem segments from the repeatedly grafted adult. The reversion was associated with appearance and disappearance of distinctive leaf proteins. Neither gibberellic acid nor N6-beneyladenine as nutrient supplements duplicated the graft effects