Genetic diversity in pepper (Capsicum spp.) by RAPD marker

• Published in: Acta horticulturae no. 9181; pp. 341 - 347
• Main Authors: Rego, E.R, Rego, M.M, Farias-Filho, L.P
• Format: Journal Article
• Language: English
• Published: International Society for Horticultural Science 2011
• Subjects: agarose; breeding; Capsicum; cluster analysis; color; cultivars; DNA; electrophoresis; genetic variation; germplasm; parents; pepper; random amplified polymorphic DNA technique; Brazil
• ISSN: 0567-7572

The Capsicum germplasm has a great diversity for morphological traits like fruit size, shape and color. This diversity offers opportunities to develop unique cultivars for agronomic applications. The study of genetic divergence is a useful and effective tool to screen accessions in germplasm banks and to identify superior parents in breeding programs. The objective of this work was to evaluate the genetic diversity among 29 accessions of the germplasm bank of the Universidade Federal de Roraima by RAPD markers. The DNA extraction was made by CTAB protocol and then submitted to amplification by RAPD analysis and separation of amplification products by agarose electrophoresis. The cluster analysis was made by UPGMA method. The DNA was extracted of the 29 accessions and amplified by 10 primers. Eight primers (UBC-135, UBC-146, UBC-155, UBC-168, UBC-228, UBC-247, UBC-253, and UBC-296) produced polymorphic bands. The minimum distance was 0.09, between AC 06 and AC 37. The accessions were grouped in ten different groups. Group 1 was the largest group formed by accessions 75, 76, 5, 32,69, 56, 73, 6, and 67; group 2 was formed by accessions 59, 72, 43, 58, and 24; groups 3 and 4 were formed by three accessions, 61, 71 and 8, 49,74, and 22, respectively; while groups 5 and 6, were formed by two accessions, 25 and 70, 45 and 55, respectively; and others group (7 to 10) were constituted by one accession (20, 44, 40,and 3, respectively). RAPD markers were effective to detect the genetic diversity among 29 hot pepper accessions.