basis of germline specificity of the hobo transposable element in Drosophila melanogaster

Previous results suggested that the hobo transposable element is active predominantly in the germline of Drosophila. We investigate germline restriction of hobo transposition by testing in vitro modified elements for their ability to mobilize marked elements in vivo. Although intact hobo elements ar...

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Bibliographic Details
Published in:	The EMBO journal Vol. 13; no. 7; pp. 1631 - 1644
Main Authors:	Calvi, B.R, Gelbart, W.M
Format:	Journal Article
Language:	English
Published:	1994
Subjects:	compound eyes DNA-binding proteins Drosophila melanogaster genetic change germ cells heat shock proteins promoter regions recombinant DNA transposase transposition transposons
Online Access:	Get full text
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Summary:	Previous results suggested that the hobo transposable element is active predominantly in the germline of Drosophila. We investigate germline restriction of hobo transposition by testing in vitro modified elements for their ability to mobilize marked elements in vivo. Although intact hobo elements are germline specific, an hsp70 promoter-hobo transposase fusion is active in the soma. Analysis of the hsp70-promoted transcript does not provide evidence for splicing. Moreover, the hobo promoter confers germline bias to a highly sensitive reporter, delta 2-3 P transposase. These results indicate that hobo transposition is germline specific due to regulation of transposase production at the level of transcription. Thus, although hobo is similar to the P transposable element in organization and tissue specificity, it differs in the underlying mechanism governing germline specific activity.
ISSN:	0261-4189 1460-2075