impact of chromosomal alteration on embryo development

• Published in: Theriogenology Vol. 65; no. 7; pp. 166 - 177
• Main Authors: King, W.A, Coppola, G, Alexander, B, Mastromonaco, G, Perrault, S, Nino-Soto, M.I, Pinto, A, Joudrey, E.M, Betts, D.H
• Format: Journal Article
• Language: English
• Published: 2006
• Subjects: chromosome aberrations; chromosome length; chromosome morphology; chromosome number; embryo (animal); embryogenesis; literature reviews; nuclear transplantation; somatic cell nuclear transfer embryos; telomerase; telomeres; X chromosome; X-inactivation
• ISSN: 0093-691X; 1879-3231

Chromosome alterations, such as those affecting telomere erosion, predictably occur with each cell division, others, which involve changes to the expression and replication of the X-chromosome occur at particular stages of development, while those that involve loss or gain of chromosomes occur in a random and so far unpredictable manner. The production of embryos in vitro and by somatic cell nuclear transfer (SCNT) has been associated with altered expression of marker genes on the X-chromosome and an increased incidence of chromosomally abnormal cells during early development. In the case of SCNT embryos chromosome abnormalities may be associated with the nuclear donor cell. Telomere rebuilding subsequent to SCNT appears to vary according to species and type of donor cell used. It is speculated that the rate of telomere erosion and incidence of chromosome abnormalities affects developmental potential of early embryos and may be potential predictors of developmental outcome.