Transdentinal protective role of sodium ascorbate against the cytopathic effects of H2 O2 released from bleaching agents

• Published in: Oral surgery, oral medicine, oral pathology, oral radiology and endodontics Vol. 109; no. 4; pp. e70 - e76
• Main Authors: Lima, Adriano Fonseca, DDS, MS, Lessa, Fernanda Campos Rosetti, DDS, MS, PhD, Gasparoto Mancini, Maria Nadir, DDS, MS, PhD, Hebling, Josimeri, DDS, MS, PhD, de Souza Costa, Carlos Alberto, DDS, MS, PhD, Marchi, Giselle Maria, DDS, MS, PhD
• Format: Journal Article
• Language: English
• Published: 2010
• Subjects: Surgery
• ISSN: 1079-2104
• DOI: 10.1016/j.tripleo.2009.12.020

Objectives The objectives of this study were to evaluate the transdentinal cytotoxicity of 10% and 16% carbamide peroxide gel (CP), as well as the ability of the antioxidant, 10% sodium ascorbate (SA), to protect the odontoblasts in culture. Study design Human dentin discs of 0.5-mm thickness were obtained and were placed into artificial pulp chambers. MDPC-23 odontoblastlike cells were seeded on pulp surface of the discs and the following groups were established: G1-No Treatment (control), G2-10% SA/6hs, G3-10%/CP6hs, G4-10%SA/6hs+10%CP/6hs, G5-16%CP/6hs, and G6-10%SA/6hs+16%CP/6hs. The cell viability was measured by the MTT assay. Results In groups where 16% CP was used, decreased cell viability was observed. Conversely, the application of 10% SA on the dentin discs, before the use of the CP, reduced the cytotoxic effects of these products on cells. Conclusions The 16% CP cause a significant decrease in MDPC-23 cell viability and 10% SA was able to partially prevent the toxic effects of CP.