Inhibitory effect of daphnetin on gastric cancer cells and its mechanism

• Published in: 精准医学杂志 Vol. 39; no. 4; pp. 328 - 332
• Main Author: ZHU Chunyang, ZHAO Shufen, WANG Yan, FANG Yuanyuan, ZHANG Siyi, QI Weiwei
• Format: Journal Article
• Language: Chinese
• Published: Editorial Office of Journal of Precision Medicine 01-08-2024
• Subjects: stomach neoplasms|daphnetin|autophagy|reactive oxygen species|membrane potential, mitochondrial
• ISSN: 2096-529X
• DOI: 10.13362/j.jpmed.202404010

Objective To explore the inhibitory effect of daphnetin (Dap) on gastric cancer cells and its mechanism. Methods Gastric cancer AGS cells were treated with Dap at concentrations of 0, 30, 60, 90, 120, and 150 μmol/L, and the effect on cell viability was evaluated after 48 h using the MTT assay. AGS cells were divided into groups A to E, and each group was treated with Dap at concentrations of 0, 30, 60, 90, 120, and 150 μmol/L, respectively, for 48 h. Each group was observed for changes in cell count and morphology using optical microscopy and crystal violet staining. DCF staining was used to evaluate the effect of Dap on intracellular reactive oxygen species (ROS) levels in AGS cells in groups A to D, while JC-1 staining was used to evaluate the effect of Dap on the level of mitochondrial membrane potential (MMP) in AGS cells in groups A to D. Western blot was conducted to measure the expression levels of LC3 and P62 proteins in AGS cells in groups A to D, and changes in autophagic flux in AGS cells were mea