Enterobacteriaceae in Gut of Honey Bee (Apis mellifera) and the Antibiotic Resistance of the Isolates

• Published in: Lucrări științifice zootehnie şi biotehnologii Vol. 50; no. 2; p. 69
• Main Authors: Jaroslav Gasper, Margarita Terentjeva, Attila Kántor, Eva Ivanišová, Vladimíra Kňazovická, Maciej Kluz, Miroslava Kačániová
• Format: Journal Article
• Language: English
• Published: Agroprint Timisoara 01-09-2023
• Subjects: antibiotic resistance; apis mellifera; intestinal microflora; maldi tof ms biotyper
• ISSN: 1841-9364; 1841-9364

Bacterial species of Enterobacteriaceae and the antimicrobial resistance of the isolates were detected in Apis mellifera L. bees gut. Gut content was cultivated on Meat peptone and McConkey agars at 30 and 37 °C, then, the isolates were identified with MALDI TOF MS Biotyper. Isolated strains were tested for antibiotic resistance to penicillins, cephalosporins, carbapenems, fluoroquinolones and aminoglycosides. Altogether, 12 species representing Enterobacteriaceae family were isolated. Firmicutes and Candida were represented by Bacillus megaterium and Issatchenkia orientalis  . Isolated Enterobacteriaceae  species were  Enterobacter cloacae, Hafnia alvei, Klebsiella oxytoca, Morganella morganii, Serratia marcescens, Ser. liquefaciens, Raoultella ornithinolytica, R. planticola, R. terrigena, Pantoea ananatis, P. agglomerans, Rahnella aquatilis. Enterobacter cloacae, Hafnia alvei, Klebsiella oxytoca, Morganella morganii, Serratia marcescens, Ser. liquefaciens isolates exhibited the antimicrobial resistance more frequently than Raoultella ornithinolytica, R. planticola, R. terrigena, Pantoea ananatis, P. agglomerans, Rahnella aquatilis. Microflora of gut of bees could serve as a source of resistant microorganisms.