Preliminary viability studies of fibroblastic cells cultured on microcrystalline and nanocrystalline diamonds produced by chemical vapour deposition method

• Published in: Materials research (São Carlos, São Paulo, Brazil) no. ahead
• Main Authors: Ana Amélia Rodrigues, Vitor Baranauskas, Helder Jose Ceragioli, Alfredo Carlos Peterlevitz, Arnaldo Rodrigues dos Santos Junior, William Dias Belangero
• Format: Journal Article
• Language: English
• Published: Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol) 01-01-2012
• Subjects: biomaterials; culture cell; CVD diamond; implants
• ISSN: 1516-1439
• DOI: 10.1590/S1516-14392012005000153

Implant materials used in orthopedics surgery have demonstrated some disadvantages, such as metallic corrosion processes, generation of wear particles, inflammation reactions and bone reabsorption in the implant region. The diamond produced through hot-filament chemical vapour deposition method is a new potential biomedical material due to its chemical inertness, extreme hardness and low coefficient of friction. In the present study we analysis two samples: the microcrystalline diamond and the nanocrystalline diamond. The aim of this study was to evaluate the surface properties of the diamond samples by scanning electron microscopy, Raman spectroscopy and atomic force microscopy. Cell viability and morphology were assessed using thiazolyl blue tetrazolium bromide, cytochemical assay and scanning electron microscopy, respectively. The results revealed that the two samples did not interfere in the cell viability, however the proliferation of fibroblasts cells observed was comparatively higher with the nanocrystalline diamond.