Effects of Two GnRH-based Recombinant Vaccine Candidates on Pig Fertility

Effects of Two GnRH-based Recombinant Vaccine Candidates on Pig Fertility

This paper describes for the first time the generation of the K88ab-GnRH hybrid fimbria, the fusion of N. meningitidis P64k protein (P64k-GnRH), and its evaluation as vaccine candidates to control fertility in mammals. Twenty hybrid male pigs were randomly distributed in four groups: placebos and im...

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Bibliographic Details
Published in:Revista de producción animal Vol. 30; no. 3; pp. 49 - 57
Main Authors: Roberto Basulto Baker, Gerardo Guillén Nieto, Lesvia Calzada Aguilera, Jesús Junco Barranco, Nelson Izquierdo Pérez, Osvaldo Reyes Acosta, Hilda Garay Pérez, Ricardo Bringas Pérez, Eulogio Pimentel Vázquez, José A. Bertot, Rafael Avilés, María Dolores Castro Santana, Eddy Bover Fuentes, Franklin Fuentes Aguilar
Format: Journal Article
Language:English
Published: Ediciones Universidad de Camagüey 01-09-2018
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Summary:This paper describes for the first time the generation of the K88ab-GnRH hybrid fimbria, the fusion of N. meningitidis P64k protein (P64k-GnRH), and its evaluation as vaccine candidates to control fertility in mammals. Twenty hybrid male pigs were randomly distributed in four groups: placebos and immunized with K88ab-GnRH, P64k-GnRH and a GnRH analogue (GnRHm1), linked to a tetanus toxoid (TT) T-helper epitope (positive control), respectively. The pigs were immunized at 9-10 weeks of age, using a two-dose scheme, and were sacrificed sixteen weeks later. K88ab-GnRH, P64k-GnRH, and GnRHm1-TT induced higher, similar, and lower testosterone levels in the serum, compared to the placebo, respectively. In the K88ab-GnRH group, the pigs underwent a reduction in testicle size and weight (P < 0.01), and a reduction in the weight of epididymes compared to the placebo; none of them was able to ejaculate. In the P64k-GnRH group, the pigs had a reduction in testicle weight (P < 0.05), and only one of them was able to ejaculate. The testicles of the pigs immunized with K88ab-GnRH and P64k-GnRH showed structural and functional damage; spermatogenesis was also affected. The accessory sexual glands of the P64k-GnRH group were normal, in contrast to K88ab-GnRH, where interstitial fibrosis was observed. The damage caused by K88ab-GnRH and P64k-GnRH in the target organs evaluated were in all cases lower than the affectations caused by the GnRHm1-TT peptide.
ISSN:2224-7920