Detection of granulocyte macrophage colony stimulating factor in quails exposed to thermal stress and treated with effective microorganism

• Published in: Iraqi journal of veterinary sciences Vol. 38; no. 3; pp. 573 - 581
• Main Authors: Marwa M. Younis, Hadil B. Al-Sabaawy
• Format: Journal Article
• Language: Arabic
• Published: University of Mosul, College of Veterinary Medicine 01-07-2024
• Subjects: heat stress; histological lesion; liver
• ISSN: 1607-3894; 2071-1255
• DOI: 10.33899/ijvs.2024.145859.3403

The study investigated the histopathological effects of the liver of quail birds prone to heat stress and treated with effective microorganisms. Forty-one-day-old broilers were erratically distributed into 4 groups, with ten birds for each group, as follows: first group as a control, second group treated with effective microorganisms, third group exposed to heat stress, and the fourth group treated with effective microorganisms and exposed to heat stress. The birds were placed in the animal house and left for 10 days to adapt. On the eleventh day, the experiment was started. For thirty days, the animals were sacrificed. On the fifteenth day and the last day of the experimentation, microscopic lesions were identified as hemorrhage, congestion, vacuole degeneration, cloudy swelling, infiltration of inflammatory cells, and necrosis. Pathological lesions were analysed statistically between the first and second slaying. They were highest in the third group and during the two. slayings. The severity of the pathological changes in the 2nd group was less than in the first, third, and fourth groups, and a significant correlation between lesions was more predominant in disturbance of circulation as hemorrhage. The intensity of protein expression represented by a granulocyte-macrophage colony stimulating factor (GMCSF) was highest in the first slaying, second, and third groups compared to the intensity of it in the fourth group at the second kill. We conclude from the results that living organisms can decrease pathological changes and be used as a protective protocol in poultry farming fields.