ATIVIDADE CELULOLÍTICA DE FUNGOS AEROBIOS ISOLADOS DO RÚMEN DE BOVINOS LEITEIROS ALIMENTADOS COM FORRAGENS TROPICAIS

• Published in: Caatinga Vol. 27; no. 4; pp. 202 - 207
• Main Authors: PATRÍCA NATALÍCIA MENDES ALMEIDA, CLÁUDIO EDUARDO SILVA FREITAS, FLÁVIA OLIVEIRA ABRÃO, IZABELLA CAROLINA OLIVEIRA RIBEIRO, EDVALDO ALVES VIEIRA, LUCIANA CASTRO GERASEEV, EDUARDO ROBSON DUARTE
• Format: Journal Article
• Language: English
• Published: Universidade Federal Rural do Semi-Árido 01-01-2014
• ISSN: 0100-316X; 1983-2125

The objective was to evaluate the cellulolytic activity of aerobic mycelian fungi from dairy cat- tle fed tropical forages. Isolates of fungi were obtained from 30 samples of rumen fluid from cows fed sorghum silage, 32 cows fed on Brachiaria brizantha , 12 heifers receiving sorghum silage and 11 heifers fed cane sugar. The isolation was performed on C solid medium containing cellulose. Were evaluated 49 isolates, 27 Aspergil- lus spp., four Gliocladium spp., six Paecilomyces spp., nine Rhizophus spp. and three Scedosporium . These isolates were incubated in C medium with 1% microcrystalline cellulose as the sole carborno source, and incu- bated at 37°C in triplicate. Cellulolytic activity indices were verified after 24, 48 and 72 hours and calculated by the ratio between degradation halo and colony diameter for comparing in nonparametric tests. The genus Aspergillus , showed higher cellulolytic activity index mean compared to Rhizophus genus (p <0.05). Eight iso- lates of Aspergillus spp. and six of Paecilomyces spp. showed this index higher than one, indicating potential for utilization in ruminant nutrition.