Bacterial endotoxins properties and methods of removing them from biopharmaceutical drugs

The production of biopharmaceutical proteins by means of Escherichia coli cultivation leads to contamination with bacterial endotoxins which are very toxic and pyrogenic impurities. The final product purification from these impurities is one of the most important tasks in the production of biopharma...

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Bibliographic Details
Published in:	Tonkie himičeskie tehnologii (Online) Vol. 9; no. 4; pp. 27 - 33
Main Authors:	A. S. Zhuravko, V. I. Shvets
Format:	Journal Article
Language:	Russian
Published:	MIREA - Russian Technological University 01-08-2014
Subjects:	recombinant proteins, protein solutions, bacterial endotoxins, lipopolysaccharides, toxic impurity
Online Access:	Get full text
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Summary:	The production of biopharmaceutical proteins by means of Escherichia coli cultivation leads to contamination with bacterial endotoxins which are very toxic and pyrogenic impurities. The final product purification from these impurities is one of the most important tasks in the production of biopharmaceutical drugs. However, isolation of proteins from cellular biomass results in the formation of strong endotoxin-protein complexes that considerably complicate the task of obtaining pharmaceutical safely drugs. The goal of the present mini-review consisted in considering and discussing effective methods of removing bacterial endotoxins from protein solutions on the basis of their chemical and biological properties.
ISSN:	2410-6593 2686-7575