DETERMINATION OF MELDONIUM, γ-BUTYROBETAINE, AND CARNITINE IN BLOOD PLASMA BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH MASS-SELECTIVE DETECTION

A procedure for simultaneous quantitative determination of meldonium, L-carnitine, and γ-butyrobetaine in blood plasma by high-performance liquid chromatography with mass-selective detection and the isotopically labeled d9L-carnitine as internal standard is proposed. An algorithm for constructing ca...

Full description

Saved in:

Bibliographic Details
Published in:	Razrabotka i registraciâ lekarstvennyh sredstv (Online) no. 1; pp. 176 - 183
Main Authors:	P. N. Sorokoumov, E. I. Savelieva, G. V. Karakashev, V. A. Kopeikin, A. S. Radilov
Format:	Journal Article
Language:	Russian
Published:	LLC Center of Pharmaceutical Analytics (LLC «CPHA») 01-01-2019
Subjects:	hplc−ms l-carnitine medonium pharmacometabolomics γ-butyrobetaine
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A procedure for simultaneous quantitative determination of meldonium, L-carnitine, and γ-butyrobetaine in blood plasma by high-performance liquid chromatography with mass-selective detection and the isotopically labeled d9L-carnitine as internal standard is proposed. An algorithm for constructing calibration plots for the quantitation of biogenic plasma components (L-carnitine, and γ-butyrobetaine) is developed. The procedure is validated for the concentration range from 0,05 to 20 µg/cm3 and applied in a pharmacometabolomic study of blood plasma on protracted administration of meldonium as a monopreparation and as a complex with L-carnitine.
ISSN:	2305-2066 2658-5049