Analysis of ancestral and functionally relevant CD5 variants in systemic lupus erythematosus patients

• Published in: PloS one
• Main Authors: Cenit, Maria Carmen, Martínez-Florensa, Mario, Consuegra, Marta, Bonet, Lizette, Carnero-Montoro, Elena, 1985, Armiger, Noelia, Caballero-Baños, Miguel, Arias, Maria Teresa, Benítez, Daniel, Ortego-Centeno, Norberto, Ramón, Enrique de, Sabio, José Mario, García-Hernández, Francisco J, Tolosa, Carles, Suárez, Ana, González-Gay, Miguel, Bosch Fusté, Elena, Martín, Javier, Lozano, Francisco
• Format: Journal Article
• Language: English
• Published: Public Library of Science (PLoS) 2014
• Subjects: Lupus eritematós sistèmic
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0113090

Objective: CD5 plays a crucial role in autoimmunity and is a well-established genetic risk factor of developing RA. Recently, evidence of positive selection has been provided for the CD5 Pro224-Val471 haplotype in East Asian populations. The aim of the present work was to further analyze the functional relevance of non-synonymous CD5 polymorphisms conforming the ancestral and the newly derived haplotypes (Pro224-Ala471 and Pro224-Val471, respectively) as well as to investigate the potential role of CD5 on the development of SLE and/or SLE nephritis. Methods: The CD5 SNPs rs2241002 (C/T; Pro224Leu) and rs2229177 (C/T; Ala471Val) were genotyped using TaqMan allelic discrimination assays in a total of 1,324 controls and 681 SLE patients of Spanish origin. In vitro analysis of CD3-mediated T cell proliferative and cytokine response profiles of healthy volunteers homozygous for the above mentioned CD5 haplotypes were also analyzed. Results: T-cell proliferation and cytokine release were significantly increased showing a bias towards to a Th2 profile after CD3 cross-linking of peripheral mononuclear cells from healthy individuals homozygous for the ancestral Pro224-Ala471 (CC) haplotype, compared to the more recently derived Pro224-Val471 (CT). The same allelic combination was statistically associated with Lupus nephritis./nConclusion: The ancestral Ala471 CD5 allele confers lymphocyte hyper-responsiveness to TCR/CD3 cross-linking and is associated with nephritis in SLE patients.
This work was supported by grants from the Spanish Ministerio de Economía y Competitividad [SAF2010-19717 to FL, SAF2009-11110 to JM, SAF2011-29239, and BFU2008-01046 to EB], Generalitat de Catalunya [2009SGR00252 to FL, and 2009SGR1101 to EB], Junta de Andalucía [CTS-4977], and Instituto de Salud Carlos III and Fondo Europeo de Desarrollo Regional/FEDER [RD12/0009/0004 to JM]