TRANSCRIPTIONAL ANALYSIS OF SOYBEAN (Glycine max) GENES RELATED TO WATER DEFICIT

• Published in: Desafios (Palmas) Vol. 11; no. 7
• Main Authors: Sérgio Bernardo Queiroz, Adriano, Clemilda Nunes, Antônia, Martins Daúde, Matheus, Gomes Barreto, Horllys, Ságio, Solange
• Format: Journal Article
• Language: English
• Published: 29-10-2024
• ISSN: 2359-3652; 2359-3652
• DOI: 10.20873/Agroenergia_2024_v11_n7_15

ABSTRACT The soybean crop has been gaining worldwide prominence, being widely disseminated due to its various forms of use in different segments. Genetic and environmental factors are decisive in the quality of this culture. A feature related to the environmental factor that has gained prominence is the water deficit. Molecular events are initiated by the perception of water stress, involving a complex genetic network. Studies in recent decades have already identified some genes related to water stress in plants, it is known that the dehydration-responsive element binding protein (DREB) plays an important role in the response to water deficit. Therefore, this work aimed to analyze the expression of genes related to water deficit in soybean. For the analysis of gene expression, leaves of soybean (Glycine max (L.) Merrill), cultivar M9144 RR, submitted to the following treatments were used: 25V25R – plants subjected to water deficit throughout the cycle, being irrigated with 25% of ETpc and 100V100R – plants that did not experience water deficit, being irrigated with 100% of ETpc throughout the cycle. The quantification of the relative expression of the genes GmDREB1 and GmDREB5 showed that the water stress suffered by the cultivar was greater in the reproductive stage. Keywords: Transcription factors, development stages, gene expression.