The SGN VIGS Tool： User-Friendly Software to Design Virus-Induced Gene Silencing （VIGS） Constructs for Functional Genomics
Dear Editor, Virus-induced gene silencing （VIGS） is a fast and powerful method to study gene function in plants （Burch-Smith et al., 2004）. It is based on plant defense mechanisms against viral gene replication and allows high-throughput silencing of genes of inter- est （SenthiI-Kumar and Mysore, 20...
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| Published in: | 分子植物：英文版 Vol. 8; no. 3; pp. 486 - 488 |
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| Main Author: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Dear Editor,
Virus-induced gene silencing （VIGS） is a fast and powerful method to study gene function in plants （Burch-Smith et al., 2004）. It is based on plant defense mechanisms against viral gene replication and allows high-throughput silencing of genes of inter- est （SenthiI-Kumar and Mysore, 2014）. The molecular mechanisms involved in post-transcriptional gene silencing （PTGS） have been studied intensively, and its steps are well known. The silencing pro- cess begins with the recognition through Dicer-like ribonucleases （DCL） of double-stranded RNA （dsRNA） that is generated during viral replication. Upon recognition, the dsRNA is processed into 21-24 nucleotide fragments, termed small interfering RNA （siRNA）. Based on sequence homology, both viral siRNA and plant mRNA associate with an RNA-induced silencing complex （RISC） and are targeted for degradation （Llave, 2010）. |
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| Bibliography: | 31-2013/Q Noe Femandez-Pozo,Hernan G. Rosli, Gregory B. Martin and Lukas A. Mueller （1 Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA ; 2 Instituto de Investigaciones Biotecnolbgicas - Instituto Tecnológico de Chascomús （IIB-INTECH）, UNSAM-CONICET, Chascomús, B71301WA Buenos Aires, Argentina;3 Section of Plant Pathology and Plant-Microbe Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, USA） Dear Editor, Virus-induced gene silencing （VIGS） is a fast and powerful method to study gene function in plants （Burch-Smith et al., 2004）. It is based on plant defense mechanisms against viral gene replication and allows high-throughput silencing of genes of inter- est （SenthiI-Kumar and Mysore, 2014）. The molecular mechanisms involved in post-transcriptional gene silencing （PTGS） have been studied intensively, and its steps are well known. The silencing pro- cess begins with the recognition through Dicer-like ribonucleases （DCL） of double-stranded RNA （dsRNA） that is generated during viral replication. Upon recognition, the dsRNA is processed into 21-24 nucleotide fragments, termed small interfering RNA （siRNA）. Based on sequence homology, both viral siRNA and plant mRNA associate with an RNA-induced silencing complex （RISC） and are targeted for degradation （Llave, 2010）. |
| ISSN: | 1674-2052 1752-9867 |