Root Extract ofPolygonum HydropiperAlters the Expression of Rat Uterine Protein Profile in Presence and Absence of Ovary in.situ during Periimplantation Period： An Evidence on SDS-PAGE

• Published in: Journal of reproduction and contraception Vol. 20; no. 4; pp. 223 - 236
• Main Author: Pranjiv Goswami Ajit Hazarika Hirendra N Sarma
• Format: Journal Article
• Language: English
• Published: 2009
• Subjects: SDS; 中药提取物; 凝胶电泳; 去卵巢; 围着床期; 子宫角; 蛋白表达
• ISSN: 1001-7844

Objective To study its effects on reproductive performance in albino rats.
Methods The chromatographic fraction （CF） of crude methanolic extract of the herb has been subcutaneously administered to female albino rats. Experiments were carried out in adult cyclic females and oavriectomised （OVX） females during early gestation period. Uterine horns were collected following the respective treatment regimen to stud）, the protein profile in 15% gel SDS-PAGE.
Results The CF induced changes in the expression of protein in rat uterus. New proteins have been expressed in uterus of adult cyclic females having ovary in-situ. The OVX females treated with CF showed altered uterine protein profile compared with that of OVX control and OVX estradiol-17β （E2） treated rats. The CF exerted its effect on expression of uterine protein during early gestation period in rats. While uterine proteins of CF treated females were similar to that of controls during preimplantation period; many of the proteins on day 6 of gestation have been found either missing or expressed in lesser intensity.
Conclusion The root of Polygonum hydropiper contains potential compound（s） which can alter the reproductive performance of female rats modulating uterine protein expression.