Effects of AIIoxan-lnduced Diabetes on the Expression of Insulin Signal rransmission Molecules

The mRNA of insulin receptor （IR）, the insulin receptor substrates （IRSs）, glucose transporter 2 （GLUT2） and glucokinase （GK） in alloxan-induced diabetic rat livers were amplified by RT-PCR. The protein of the insulin receptor in rat livers was determined by westem-blotting. The results show that IR...

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Bibliographic Details
Published in:	Wuhan University journal of natural sciences no. 5; pp. 447 - 451
Main Author:	GE Yili PENG Hong SHENG Xiqun ZHANG Gongchen ZHOU Jun WANG Miao ZHOU Xiaoping HUANG Kaixun
Format:	Journal Article
Language:	English
Published:	2009
Subjects:	分子表达四氧嘧啶大鼠肝脏糖尿病大鼠胰岛素受体底物葡萄糖转运蛋白蛋白水平
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Summary:	The mRNA of insulin receptor （IR）, the insulin receptor substrates （IRSs）, glucose transporter 2 （GLUT2） and glucokinase （GK） in alloxan-induced diabetic rat livers were amplified by RT-PCR. The protein of the insulin receptor in rat livers was determined by westem-blotting. The results show that IR expression level decreased at the level of mRNA and protein. The gene expressions of IRSs, GLUT2 and GK changed significantly. The hepatic glycogen content in alloxan-diabetic rats treated with insulin （（13.2 ± 0.4） mg·g^-1） did not restore to normal level （（17.0 ±0.4） mg·g^-1） by means of anthrone-H2SO4 methods. The results imply that insulin resistance is developed during inchoate phase of alloxan-induced diabetic rats.
Bibliography:	Type I diabetes Q578 alloxan; Type I diabetes; insulin signal transmission; RT-PCR; Western-blotting insulin signal transmission RT-PCR Western-blotting alloxan 42-1405/N T-332
ISSN:	1007-1202 1993-4998