Induction of phagocytic activity of M1 cells by an inhibitor of vacuolar FT‐ATPase, bafilomycin A1

• Published in: FEBS letters Vol. 337; no. 3; pp. 221 - 225
• Main Authors: Kinoshita, Kuninori, Hidaka, Hiroyoshi, Ohkuma, Shoji
• Format: Journal Article
• Language: English
• Published: 17-01-1994
• Subjects: Bafilomycin a1; Leukemia inhibitory factor; Macrophage; Phagocytic activity; Vacuolar H+-ATPase
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(94)80195-9

Bafilomycin a1, a selective inhibitor of vacuolar H+‐ATPase, time‐and dose‐dependently induced the differentiation of M1 cells, a murine myeloid leukemic cell line, into macrophage‐like cells as revealed by the phagocytosis of polystyrene latex particles. This differentiation was inhibited not only by actinomycin D and cycloheximide but also by ST‐638 (an inhibitor of tyrosine kinase). However, it was affected neither by K‐252a (an inhibitor of C‐kinase) nor by H‐89 (an inhibitor of A‐kinase), in contrast to the M1 cell differentiation induced by leukemia inhibitory factor (LIF). Okadaic acid inhibited both the bafilomycin A1‐induced and LIF‐induced differentiation of M1 cells.