Detecting long-term metabolic shifts using isotopomers CO2-driven suppression of photorespiration in C-3 plants over the 20th century

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 112; no. 51; p. 15585
• Main Authors: Ehlers, Ina, Augusti, Angela, Betson, Tatiana R., Nilsson, Mats B., Marshall, John D., Schleucher, Juergen
• Format: Journal Article
• Language: English
• Published: 2015
• Subjects: acclimation; atmospheric change; CO2 fertilization; deuterium; Forest Science; isotopomer; Skogsvetenskap
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.1504493112

Terrestrial vegetation currently absorbs approximately a third of anthropogenic CO2 emissions, mitigating the rise of atmospheric CO2. However, terrestrial net primary production is highly sensitive to atmospheric CO2 levels and associated climatic changes. In C-3 plants, which dominate terrestrial vegetation, net photosynthesis depends on the ratio between photorespiration and gross photosynthesis. This metabolic flux ratio depends strongly on CO2 levels, but changes in this ratio over the past CO2 rise have not been analyzed experimentally. Combining CO2 manipulation experiments and deuterium NMR, we first establish that the intramolecular deuterium distribution (deuterium isotopomers) of photosynthetic C-3 glucose contains a signal of the photorespiration/photosynthesis ratio. By tracing this isotopomer signal in herbarium samples of natural C-3 vascular plant species, crops, and a Sphagnum moss species, we detect a consistent reduction in the photorespiration/photosynthesis ratio in response to the similar to 100-ppm CO2 increase between similar to 1900 and 2013. No difference was detected in the isotopomer trends between beet sugar samples covering the 20th century and CO2 manipulation experiments, suggesting that photosynthetic metabolism in sugar beet has not acclimated to increasing CO2 over >100 y. This provides observational evidence that the reduction of the photorespiration/photosynthesis ratio was ca. 25%. The Sphagnum results are consistent with the observed positive correlations between peat accumulation rates and photosynthetic rates over the Northern Hemisphere. Our results establish that isotopomers of plant archives contain metabolic information covering centuries. Our data provide direct quantitative information on the "CO2 fertilization" effect over decades, thus addressing a major uncertainty in Earth system models.