Distribution of reaction products in phospholipase A(2) hydrolysis

• Published in: Biochimica et biophysica acta. Biomembranes Vol. 1768; no. 5; p. 1036
• Main Authors: Wacklin, Hanna, Tiberg, Fredrik, Fragneto, Giovanna, Thomas, Robert K.
• Format: Journal Article
• Language: English
• Published: 01-05-2007
• Subjects: AIR/WATER INTERFACE; Chemical Sciences; COMPONENT VOLUMES; DODECYL MALTOSIDE; fatty acid; FATTY-ACIDS; Fysikalisk kemi; Kemi; lag phase; LIPID-BILAYERS; lyso-lipid; Natural Sciences; Naturvetenskap; neutron reflection SILICA-WATER INTERFACE; NEUTRON REFLECTIVITY; phospholipase A; Physical Chemistry; STRUCTURAL-CHANGES; supported bilayer; UNILAMELLAR VESICLES; X-RAY
• ISSN: 0005-2736
• DOI: 10.1016/j.bbamem.2006.10.020

We have monitored the composition of supported phospholipid bilayers during phospholipase A(2) hydrolysis using specular neutron reflection and ellipsometry. Porcine pancreatic PLA(2) shows along lag phase of several hours during which the enzyme binds to the bilayer surface, but only 5 +/- 3% of the lipids react before the onset of rapid hydrolysis. The amount of PLA(2), which resides in a 21 +/- 1 angstrom thick layer at the water-bilayer interface, as well as its depth of penetration into the membrane, increase during the lag phase, the length of which is also proportional to the enzyme concentration. Hydrolysis of a single-chain deuterium labelled d(31)-POPC reveals for the first time that there is a significant asymmetry in the distribution of the reaction products between the membrane and the aqueous environment. The lyso-lipid leaves the membrane while the number of PLA(2) Molecules bound to the interface increases with increasing fatty acid content. These results constitute the first direct measurement of the membrane structure and composition, including the location and amount of the enzyme during hydrolysis. These are discussed in terms of a model of fatty-acid mediated activation of PLA(2).