Relation between the insulin receptor number in cells, autophosphorylation and insulin-stimulated Ras-GTP formation

We showed previously that upon insulin stimulation of an insulin receptor overexpressing cell line, most of the p21ras was rapidly converted into the GTP bound state. To determine whether this process also occurs in cells expressing physiologically relevant numbers of insulin receptors, insulin stim...

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Bibliographic Details
Published in:	The Journal of biological chemistry Vol. 267; no. 21; pp. 14647 - 14653
Main Authors:	Osterop, APRM, Medema, R H, Bos, J L, v. d. Zon, GCM, Moller, DE, Flier, J S, Moeller, W, Maasen, JA
Format:	Journal Article
Language:	English
Published:	01-01-1992
Subjects:	correlation insulin phosphorylation protein-tyrosine kinase Ras protein receptors stimulation
Online Access:	Get full text
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Summary:	We showed previously that upon insulin stimulation of an insulin receptor overexpressing cell line, most of the p21ras was rapidly converted into the GTP bound state. To determine whether this process also occurs in cells expressing physiologically relevant numbers of insulin receptors, insulin stimulated Ras-GTP formation was quantitated in Chinese hamster ovary (CHO)-derived cell lines expressing varying numbers of insulin receptors.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0021-9258