Serum-Free and Protein-Free Media for the Cultivation of Recombinant Chinese Hamster Ovary (CHO) Cell Lines

We describe the preparation of two cell culture media formulations for the culture in suspension of Chinese hamster ovary (CHO) cell lines. The first medium, Cell growth SFM Medium, is a serum-free medium designed to maintain cell growth with high-viability profiles. The second corresponds to a prot...

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Bibliographic Details
Published in:	Methods in molecular biology (Clifton, N.J.) Vol. 2853; p. 1
Main Authors:	Capella Roca, Berta, Meiller, Justine, Doolan, Padraig, Clynes, Martin
Format:	Journal Article
Language:	English
Published:	United States 2025
Subjects:	Animals Cell Culture Techniques - methods Cell Proliferation - drug effects CHO Cells Cricetinae Cricetulus Culture Media - chemistry Culture Media, Serum-Free Recombinant Proteins - genetics Recombinant Proteins - metabolism Recombinant Chinese hamster ovary cells Protein-free media Serum-free media
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Summary:	We describe the preparation of two cell culture media formulations for the culture in suspension of Chinese hamster ovary (CHO) cell lines. The first medium, Cell growth SFM Medium, is a serum-free medium designed to maintain cell growth with high-viability profiles. The second corresponds to a protein-free version optimized to increase CHO recombinant protein production (Production PFM Medium). Considerations on the use of these formulations for other cell culture assays (such as transfections) are also described.
ISSN:	1940-6029
DOI:	10.1007/978-1-0716-4104-0_1