MiR-24 improves beta-like globin gene expression through targeting Sp1

We studied the function and mechanism of miR-24 in regulating beta-like globin gene expression. We first detected the expression of miR-24 during erythroid differentiation and also detected the globin gene expression in miR-24 overexpressing K562 cells through q-PCR. Dual-luciferase reporter assay a...

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Bibliographic Details
Published in:	Shengwu gongcheng xuebao Vol. 29; no. 7; p. 946
Main Authors:	Ma, Yanni, Wang, Bin, Gong, Bei, Wang, Fang, Zhao, Hualu, Zhang, Junwu, Yu, Jia
Format:	Journal Article
Language:	Chinese
Published:	China 01-07-2013
Subjects:	Cell Differentiation epsilon-Globins - genetics gamma-Globins - genetics Gene Expression Regulation Hematopoietic Stem Cells - metabolism Humans K562 Cells MicroRNAs - genetics Sp1 Transcription Factor - genetics
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Summary:	We studied the function and mechanism of miR-24 in regulating beta-like globin gene expression. We first detected the expression of miR-24 during erythroid differentiation and also detected the globin gene expression in miR-24 overexpressing K562 cells through q-PCR. Dual-luciferase reporter assay and Western blotting were used to identify target genes of miR-24. "Rescue experiment" was further used to investigate the regulation of miR-24 on globin gene expression whether depending on targeting Sp1 or not. We found that miR-24 increased during hemin-induced K562 cells and EPO-induced HPCs (hematopoietic progenitor cells) erythroid differentiation. Overexpression of miR-24 in K562 cells promoted the epsilon- and gamma-globin gene expression during hemin-induced erythroid differentiation through targeting the negative globin regulator Sp1. These results suggested that miR-24 can improve the expression of beta-like globin gene through targeting Sp1.
ISSN:	1000-3061