Salicylic Acid, an Ambimobile Molecule Exhibiting a High Ability to Accumulate in the Phloem1

• Published in: Plant physiology (Bethesda) Vol. 141; no. 4; pp. 1684 - 1693
• Main Authors: Rocher, Françoise, Chollet, Jean-François, Jousse, Cyril, Jean-Louis Bonnemain
• Format: Journal Article
• Language: English
• Published: Rockville American Society of Plant Biologists 01-08-2006
• Subjects: Liquid chromatography; Seedlings; Spectrometry
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.106.082537

The ability of exogenous salicylic acid (SA) to accumulate in castor bean (Ricinus communis) phloem was evaluated by HPLC and liquid scintillation spectrometry analyses of phloem sap collected from the severed apical part of seedlings. Time-course experiments indicated that SA was transported to the root system via the phloem and redistributed upward in small amounts via the xylem. This helps to explain the peculiarities of SA distribution within the plant in response to biotic stress and exogenous SA application. Phloem loading of SA at 1, 10, or 100 microm was dependent on the pH of the cotyledon incubating solution, and accumulation in the phloem sap was the highest (about 10-fold) at the most acidic pH values tested (pH 4.6 and 5.0). As in animal cells, SA uptake still occurred at pH values close to neutrality (i.e. when SA is only in its dissociated form according to the calculations made by ACD LogD suite software). The analog 3,5-dichlorosalicylic acid, which is predicted to be nonmobile according to the models of Bromilow and Kleier, also moved in the sieve tubes. These discrepancies and other data may give rise to the hypothesis of a possible involvement of a pH-dependent carrier system translocating aromatic monocarboxylic acids in addition to the ion-trap mechanism.