Effects of [Beta]-Cyclodextrin on the Structure of Sphingomyelin/Cholesterol Model Membranes

• Published in: Biophysical journal Vol. 99; no. 5; pp. 1475 - 1481
• Main Authors: Jablin, Michael S, Flasinski, Michal, Dubey, Manish, Ratnaweera, Dilru R, Broniatowski, Marcin, Dynarowicz-Latka, Patrycja, Majewski, Jaroslaw
• Format: Journal Article
• Language: English
• Published: New York Biophysical Society 08-09-2010
• Subjects: Asymmetry; Biophysics; Cholesterol; Complexation; Deposition; Membranes; Molecules; Packing density; Scattering; Surface pressure
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1016/j.bpj.2010.06.028

The interaction of β-cyclodextrin (β-CD) with mixed bilayers composed of sphingomylein and cholesterol (Chol) above and below the accepted stable complexation ratio (67:33) was investigated. Membranes with the same (symmetric) and different (asymmetric) compositions in their inner and outer leaflets were deposited at surface pressures of 20, 30, and 40 mN/m at the solid-liquid interface. Using neutron reflectometry, membranes of various global molar ratios (defined as the sum of the molar ratios of the inner and outer leaflets), were characterized before and after β-CD was added to the subphase. The structure of bilayers with global molar ratios at or above the stable complexation ratio was unchanged by β-CD, indicating that β-CD is unable to remove sphingomyelin or complexed Chol. However, β-CD removed all uncomplexed Chol from bilayers composed of global molar ratios below the stable complexation ratio. The removal of Chol by β-CD was independent of the initial structure of the membranes as deposited, suggesting that asymmetric membranes homogenize by the exchange of molecules between leaflets. The interaction of β-CD with the aforementioned membranes was independent of the deposition surface pressure except for a symmetric 50:50 membrane deposited at 40 mN/m. The scattering from 50:50 bilayers with higher packing densities (deposited at 40 mN/m) was unaffected by β-CD, suggesting that the removal of Chol can depend on both the composition and packing density of the membrane. [PUBLICATION ABSTRACT]