Development of methods for rapid test of staphylococcal enterotoxin A in food

Noninstrumental methods of qualitative rapid test for detection of staphylococcal enterotoxin A (SEA) in milk foods sample and brothof using immunochromatography (IC) and dot-assay has been developed. Monoclonal antibodies to SEA with colloidal gold forimmunochromatography; monoclonal antibodies to...

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Bibliographic Details
Published in:	Bioorganicheskaia khimiia Vol. 40; no. 2; p. 186
Main Authors:	Liubavina, I A, Brovko, F A, Valiakina, T I, Vertiev, Iu V, Grishin, E V
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-03-2014
Subjects:	Animals Antibodies, Monoclonal Enterotoxins - chemistry Enterotoxins - isolation & purification Enzyme-Linked Immunosorbent Assay - methods Food Analysis - methods Gold Colloid - chemistry Humans Meat Products - microbiology Milk - microbiology Streptavidin - chemistry
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Summary:	Noninstrumental methods of qualitative rapid test for detection of staphylococcal enterotoxin A (SEA) in milk foods sample and brothof using immunochromatography (IC) and dot-assay has been developed. Monoclonal antibodies to SEA with colloidal gold forimmunochromatography; monoclonal antibodies to SEA with colloidal gold or biotinylated monoclonal antibodies and streptavidin-peroxidase conjugate for dot-assay were used to visualize the results. The detection limits, ng/mL: 10 (IC), 20 (dot-assay with antibody-colloidal gold), 10 (dot-assay with STR-HRP), 4 (ELISA). Time of assay, min: 25 (IC), 60 (dot-assay with antibody-colloidal gold), 70 (dot-assay with STR-HRPO, 150 (ELISA).
ISSN:	0132-3423