Role of glucocorticoids and capsaicin-sensitive neurons in gastroprotective effect of ischemic preconditioning

• Published in: Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova Vol. 98; no. 10; p. 1242
• Main Authors: Bagaeva, T P, Bobryshev, P Iu, Komkova, O P, Filaretova, L P
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-10-2012
• Subjects: Adrenalectomy; Animals; Capsaicin - metabolism; Corticosterone - antagonists & inhibitors; Corticosterone - metabolism; Corticosterone - physiology; Ischemic Preconditioning; Male; Metyrapone - pharmacology; Neurons - drug effects; Neurons - physiology; Rats; Reperfusion Injury - physiopathology; Stomach - blood supply; Stomach - drug effects; Stomach - physiopathology; Stomach Ulcer - metabolism; Stomach Ulcer - physiopathology
• ISSN: 0869-8139

The study was designed to investigate contribution of glucocorticoid hormones and capsaicin-sensitive neurons to gastroprotective effect of ischemic preconditioning against injury induced by 3.5 h gastric ischemia-reperfusion in rats. The contribution of glucocorticoids was investigated by an inhibition of corticosterone synthesis with metyrapone and adrenalectomy followed by corticosterone replacement. To estimate the role of capsaicin-sensitive neurons we used their desensitization. Gastric ischemia was performed by occlusion of celiac artery in anaesthetized rats. In the control animals gastric ischemic preconditioning (0.5 h ischemia-reperfusion) induced plasma corticosterone rise and attenuated gastric injury caused by 3.5 h ischemia-reperfusion. Both adrenalectomy and desensitization of capsaicin-sensitive neurons by itself aggravated ulcerogenic influence of 3.5 h gastric ischemia-reperfusion. However, only adrenalectomy or metyrapone pretreatment, but not desensitization ofcapsaicin-sensitive neurons prevented gastroprotective effect ofischemic preconditioning. The data suggest that glucocorticoids contribute to gastroprotective effect of ischemic preconditioning even in rats with desensitization of capsaicin-sensitive neurons. The findings support participation of capsaicin-sensitive neurons in gastroprotection against injury caused by prolonged ischemia-reperfusion, but not in gastroprotective effect ofischemic preconditioning.