Development and validation of a capillary electrophoresis method with capacitively coupled contactless conductivity detection (CE-C(4) D) for the analysis of amikacin and its related substances

• Published in: Electrophoresis Vol. 33; no. 17; pp. 2777 - 2782
• Main Authors: El-Attug, Mohamed Nouri, Adams, Erwin, Van Schepdael, Ann
• Format: Journal Article
• Language: English
• Published: Germany 01-09-2012
• Subjects: Amikacin - analogs & derivatives; Amikacin - analysis; Amikacin - chemistry; Electric Conductivity; Electrophoresis, Capillary - methods; Hydrogen-Ion Concentration; Linear Models; Osmolar Concentration; Regression (Psychology); Reproducibility of Results; Sensitivity and Specificity
• ISSN: 1522-2683
• DOI: 10.1002/elps.201100688

Amikacin is a semisynthetic aminoglycoside antibiotic derived from kanamycin A that lacks a strong UV absorbing chromophore or fluorophore. Due to the physicochemical properties of amikacin and its related substances, CE in combination with capacitively coupled contactless conductivity detection (CE-C(4) D) was chosen. The optimized separation method uses a BGE composed of 20 mM MES adjusted to pH 6.6 by l-histidine and 0.3 mM CTAB that was added as flow modifier in a concentration below the CMC. Ammonium acetate 20 mg.L(-1) was used as internal standard. 30 kV was applied in reverse polarity on a fused silica capillary (73/48 cm; 75 μm id). The optimized separation was obtained in less than 6 min with good linearity (R(2) = 0.9996) for amikacin base. It shows a good precision expressed as RSD on relative peak areas equal to 0.1 and 0.7% for intraday and interday, respectively. The LOD and LOQ are 0.5 mg.L(-1) and 1.7 mg.L(-1) , respectively.