Chronic exposure of biomass ambient particulate matter induces signal-regulatory protein α (SIRPα) and surfactant protein D expression in rat alveolar macrophages

• Published in: Zhonghua jiehe he huxi zazhi Vol. 44; no. 12; p. 1090
• Main Authors: Wang, S L, Li, B, Ran, P X
• Format: Journal Article
• Language: Chinese
• Published: China 12-12-2021
• Subjects: Animals; Biomass; Macrophages, Alveolar; Male; Particulate Matter - toxicity; Pulmonary Surfactant-Associated Protein D; Rats; Rats, Sprague-Dawley
• ISSN: 1001-0939
• DOI: 10.3760/cma.j.cn112147-20210406-00230

To observe the expression of signal-regulatory protein α (SIRPα), surfactant protein (SP) A, and SPD by, and the phagocytic function of alveolar macrophages (AMs) in the rats exposed to biomass ambient particulate matter (BMF).
Seventy-two male SD rats were randomly divided into BMF group and clean air group. Protein levels of SIRPα, SPA, and SPD in AMs were determined by Western blotting and immunofluorescent assay after 4 days, 1 month, and 6 months of BMF exposure. Fluorescent labeled
and
were used to detect the phagocytic ability of AMs in rats at three time points.
After 4 days of BMF exposure, there was no significant difference in the protein levels of SIRPα and SPD compared with the clean air group (
>0.05). The relative levels of SIRPα and SPD were (1.73±0.64) and (2.01±0.78) at 1 month of BMF exposure, and those at 6 months of BMF exposure were (1.49±0.28) and (1.48±0.34), both of which were higher than those in the clean air group (
0.05). The relative level of
median fluorescence intensity (MFI) a