Obtaining the transgenic lines of finger millet Eleusine coracana (L.) Gaertn. With dinitroaniline resistance

The current data is dedicated to the study of bioballistic and Agrobacterium-mediated transformation of finger millet with the constructs carrying the mutant alpha-tubulin gene (TUAm 1), isolated from R-biotype goosegrass (Eleusine indica L.), for the decision of problem of dinitroaniline-resistance...

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Bibliographic Details
Published in:	TSitologiia i genetika Vol. 48; no. 3; p. 3
Main Authors:	Baer, G Ia, Emets, A I, Blium, Ia B
Format:	Journal Article
Language:	Russian
Published:	Ukraine 01-05-2014
Subjects:	Eleusine - drug effects Eleusine - genetics Eleusine - growth & development Genes, Plant Genetic Vectors Herbicide Resistance - genetics Herbicides - pharmacology Heterozygote Plants, Genetically Modified Transformation, Genetic Trifluralin - pharmacology Tubulin - genetics
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Summary:	The current data is dedicated to the study of bioballistic and Agrobacterium-mediated transformation of finger millet with the constructs carrying the mutant alpha-tubulin gene (TUAm 1), isolated from R-biotype goosegrass (Eleusine indica L.), for the decision of problem of dinitroaniline-resistance. It was found that 10 microM of trifluralin is optimal for the selection of transgene plants of finger millet. PCR analysis of transformed lines confirmed the transgene nature of plants. The analysis of seed of T1 oftransgene lines confirmed heterozygous character of inheritance of the resistance.
ISSN:	0564-3783