Real-time PCR kits for the detection of the African Swine Fever virus

• Published in: Voprosy virusologiĭ Vol. 59; no. 5; p. 47
• Main Authors: Latyshev, O E, Eliseeva, O V, Grebennikova, T V, Verkhovskiĭ, O A, Tsibezov, V V, Chernykh, O Iu, Dzhailidi, G A, Aliper, T I
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 2014
• Subjects: African Swine Fever - diagnosis; African Swine Fever - virology; African Swine Fever Virus - genetics; Animals; Antigens, Viral - immunology; DNA Primers - chemical synthesis; DNA Probes - chemical synthesis; DNA, Viral - genetics; DNA, Viral - isolation & purification; Enzyme-Linked Immunosorbent Assay; Reagent Kits, Diagnostic; Real-Time Polymerase Chain Reaction - standards; Real-Time Polymerase Chain Reaction - veterinary; Sensitivity and Specificity; Swine
• ISSN: 0507-4088

The results obtained using the diagnostic kit based on real-time polymerase chain reaction to detect the DNA of the African Swine Fever in the pathological material, as well as in the culture fluid, are presented. A high sensitivity and specificity for detection of the DNA in the organs and tissues of animals was shown to be useful for detection in the European Union referentiality reagent kits for DNA detection by real time PCR of ASFV. More rapid and effective method of DNA extraction using columns mini spin Quick gDNA(TM) MiniPrep was suggested and compared to the method of DNA isolation on the inorganic sorbent. High correlation of the results of the DNA detection of ASFV by real-time PCR and antigen detection results ASFV by competitive ELISA obtained with the ELISA SEROTEST/INGEZIM COMRAC PPA was demonstrated. The kit can be used in the veterinary services for effective monitoring of ASFV to contain, eliminate and prevent further spread of the disease.