Colonic morphological changes in experimental chronic colitis induced by dextran sulfate sodium

• Published in: Arkhiv patologii Vol. 76; no. 4; p. 29
• Main Authors: Zolotova, N A, Kiriukhin, S O, Khochansky, D N, Dobrynina, M T, Makarova, O V
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-07-2014
• Subjects: Animals; Chronic Disease; Colitis - chemically induced; Colitis - pathology; Colon - pathology; Dextran Sulfate - toxicity; Disease Models, Animal; Humans; Intestinal Mucosa - pathology; Lymphocytes - pathology; Male; Mice
• ISSN: 0004-1955

To characterize morphological changes in the colonic wall in experimental chronic colitis induced by dextran sulfate sodium (DSS).
Experiments were carried out on sexually mature male C57B1/6 mice. Drinking water in the animal dishes was replaced by 2% DSS solution for 6 days and the animals were taken away from the experiment on day 21 after initiation of DSS consumption. Hematoxylin- and eosin-stained colonic histological specimens were prepared by Masson's trichrome and Nissl methods.
Histologically, the colonic wall displayed epithelialized ulcers; mucosal remodeling; reductions in the count of goblet cells, crypts and their depth; mucosal inflammatory infiltration with lymphocytes, plasmocytes, and histiocytes; lymphoid tissue hyperplasia; diffuse and focal sclerosis of all colonic membranes; neuronal destructive changes in the myenteric and submucosal nerve plexuses.
The experimental model of DSS-induced chronic colitis corresponds to human colitis in morphological characteristics to a certain degree and may be used to study of the pathogenesis of this disease and to preclinically evaluate of the efficacy of medications.