Effects of dietary fat level on the xenobiotic metabolism enzymes activity and antioxidant enzymes in rats

• Published in: Voprosy pitaniia Vol. 81; no. 1; p. 24
• Main Authors: Kravchenko, L V, Aksenov, I V, Trusov, N V, Guseva, G V, Avren'eva, L I
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 2012
• Subjects: Animals; Antioxidants - metabolism; Aryl Hydrocarbon Hydroxylases - analysis; Aryl Hydrocarbon Hydroxylases - metabolism; Aryldialkylphosphatase - blood; Aryldialkylphosphatase - metabolism; Catalase - analysis; Catalase - metabolism; Cytochrome P-450 CYP1A1 - analysis; Cytochrome P-450 CYP1A1 - metabolism; Cytochrome P-450 CYP1A2 - analysis; Cytochrome P-450 CYP1A2 - metabolism; Cytochrome P-450 CYP2B1 - analysis; Cytochrome P-450 CYP2B1 - metabolism; Cytochrome P-450 CYP3A - analysis; Cytochrome P-450 CYP3A - metabolism; Diet, Fat-Restricted; Dietary Fats - administration & dosage; Dietary Fats - analysis; Dietary Fats - metabolism; Glucuronosyltransferase - analysis; Glucuronosyltransferase - metabolism; Glutathione Peroxidase - analysis; Glutathione Peroxidase - metabolism; Glutathione Reductase - analysis; Glutathione Reductase - metabolism; Glutathione Transferase - analysis; Glutathione Transferase - metabolism; Heme Oxygenase-1 - analysis; Heme Oxygenase-1 - metabolism; Liver - enzymology; Liver - metabolism; Male; Malondialdehyde - blood; Malondialdehyde - metabolism; Microsomes, Liver - enzymology; Plant Oils - administration & dosage; Plant Oils - metabolism; Rats; Rats, Wistar; Sunflower Oil
• ISSN: 0042-8833

Male Wistar rats received fat-free diet or diets containing 5, 10 and 30% of fat (sunflower oil + lard, 1:1) for 4 weeks. The direct relationship between dietary fat level and ethoxyresorufin O-dealkylase activity of CYP1A1, methoxyresorufin O-dealkylase activity of CYP1A2, pentoxyresorufin O-dealkylase activity of CYP2B1 and testosterone 6beta-hydroxylase activity of CYP3A was found. Activities of key enzymes of phase II xenobiotic metabolism (total activity of glutathione transferase, activity of UDP-glucuronosyle transferase) and antioxidant enzymes (catalase, glutathione peroxidase, glutathione reductase, paraoxonase-1 and heme oxygenase-1) also increased with higher dietary fat level.