Expression and significance of microtubule associated protein 1 light chain 3B, p62 and Beclin1 in lesion tissues of oral lichen planus patients

• Published in: Zhonghua kouqiang yixue zazhi Vol. 57; no. 12; p. 1217
• Main Authors: Shen, M X, Hu, W Y, Cai, Y
• Format: Journal Article
• Language: Chinese
• Published: China 09-12-2022
• Subjects: Autophagy; Beclin-1; Humans; Lichen Planus, Oral - metabolism; Microtubule-Associated Proteins - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism
• ISSN: 1002-0098
• DOI: 10.3760/cma.j.cn112144-20220327-00136

To explore the expression of autophagy related factors microtubule associated protein 1 light chain 3B (LC3B), p62, autophagy key factor Beclin1 in oral lichen planus (OLP) tissues and their relationships with the clinicopathological characteristics of OLP, investigating the function and significance of autophagy in pathogenesis of OLP.
Forty-one lesion tissues (OLP group, twenty-one cases of erosive OLP and twenty cases of non-erosive OLP) were selected from OLP patients visiting the Department of Periodontal and Oral Medicine, School and Hospital of Stomatology, Guizhou Medical University from October 2017 to December 2019. Fifteen cases of normal oral mucosal tissues (control group) were collected from oral and maxillofacial surgery at The Affiliated Stomatology Hospital of Guizhou Medical University during the same period. Protein and mRNA expression levels of LC3B, p62 and Beclin1 were detected by immunohistochemistry (IHC) and real-time quantitative PCR (RT-qPCR) in OLP lesions respectively. The protein