Trans fatty acid-induced NF-kappaB activation does not induce insulin resistance in cultured murine skeletal muscle cells

• Published in: Lipids Vol. 45; no. 3; p. 285
• Main Authors: Hommelberg, Pascal P H, Langen, Ramon C J, Schols, Annemie M W J, van Essen, Anon L M, Snepvangers, Frank J M, Mensink, Ronald P, Plat, Jogchum
• Format: Journal Article
• Language: English
• Published: United States 01-03-2010
• Subjects: Animals; Cells, Cultured; Insulin Resistance - physiology; Mice; Muscle, Skeletal - cytology; Muscle, Skeletal - metabolism; NF-kappa B - metabolism; Rats; Trans Fatty Acids - chemistry; Trans Fatty Acids - metabolism
• ISSN: 1558-9307
• DOI: 10.1007/s11745-010-3388-1

Long-chain saturated fatty acids such as palmitic acid induce insulin resistance and NF-kappaB activation in skeletal muscle cells. Here we investigated the effects of long-chain fatty acid (FA) saturation and configuration on NF-kappaB activity and insulin sensitivity in cultured skeletal muscle cells. Of all tested unsaturated FAs, only elaidic acid (3-fold), cis9,trans11-CLA (3-fold) and trans10,cis12-CLA (13-fold) increased NF-kappaB transactivation in myotubes. This was not accompanied by decreased insulin sensitivity (measured as insulin-induced glucose uptake and GLUT4 translocation). We therefore conclude that FA-induced NF-kappaB activation is not sufficient for the induction of insulin resistance in skeletal muscle cells.